منابع مشابه
Flexible structured illumination microscope with a programmable illumination array.
Structured illumination microscopy (SIM) has grown into a family of methods which achieve optical sectioning, resolution beyond the Abbe limit, or a combination of both effects in optical microscopy. SIM techniques rely on illumination of a sample with patterns of light which must be shifted between each acquired image. The patterns are typically created with physical gratings or masks, and the...
متن کاملDevelopment and testing of an AO-structured illumination microscope
The design of an Adaptive Optics (AO) Structured Illumination (SI) microscope is presented. Two key technologies are combined to provide effective super-resolution at significant depths in tissue. AO is used to measure and compensate for optical aberrations in both the system and the tissue by measuring the optical path differences in the wavefront. Uncorrected, these aberrations significantly ...
متن کاملA Structured Illumination Method for Microscope Stage Tracking
This paper explores a new application of structured illumination to determine the position of a microscope stage. Measuring stage position accurately is important for any experiment that spans multiple fields of view. The structured illumination pattern is provided by a design printed on the microscope slide or cover slip that modulates the image of the specimen. A novel application of frequenc...
متن کاملOblique Illumination Stereoscopy in the Shadow Electron Microscope
The point projection or shadow electron microscope [1] is primarily used as a convergent-beam electron diffraction camera [2]. In this mode the specimen is at the same height as the convergent-beam (C.B.) source, i.e., the image of the original source which is formed in the vicinity of the back focal plane of the objective lens. The complimentary operation of the instrument as an underfocussed ...
متن کاملAn inexpensive programmable illumination microscope with active feedback.
We have developed a programmable illumination system capable of tracking and illuminating numerous objects simultaneously using only low-cost and reused optical components. The active feedback control software allows for a closed-loop system that tracks and perturbs objects of interest automatically. Our system uses a static stage where the objects of interest are tracked computationally as the...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nature
سال: 1922
ISSN: 0028-0836,1476-4687
DOI: 10.1038/109078a0